Dilution Factor: 5 mL into 50 mL
5 mL diluted to a final volume of 50 mL produces a dilution factor of 10.
How to use this tool
- Enter aliquot volume (sample taken) and final volume after dilution in the fields above.
- Results update instantly as you type — or click Calculate.
- Read your dilution factor (df) and the full breakdown beneath it.
Taking a 5 mL aliquot and diluting it to 50 mL total volume results in a 10-fold dilution factor.
Frequently asked questions
- How is this different from the C₁V₁ dilution calculator?
- C₁V₁ = C₂V₂ calculates concentrations. The dilution factor calculator focuses on the volumetric ratio used in serial dilutions, microbiology, and spectroscopy where you track fold-dilution rather than absolute molarity.
- What is a serial dilution?
- Repeated dilutions where each step uses the previous diluted solution as input. Three 1:10 dilutions gives 1:1000 overall (DF = 1000).