AbraCalc

Beer-Lambert Law: Absorbance 0.5, ε = 1000 L/mol·cm, 1 cm Path

With molar absorptivity of 1000 L/mol·cm, absorbance of 0.5, and a 1 cm path length, the concentration is 0.0005 mol/L.

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  1. Enter absorbance a, molar absorptivity ε, concentration c and path length l in the fields above.
  2. Results update instantly as you type — or click Calculate.
  3. Read your absorbance a and the full breakdown beneath it.

Use the Beer-Lambert law to find concentration from an absorbance reading of 0.5 with ε = 1000 L/mol·cm and a standard 1 cm cuvette path length.

Frequently asked questions

What is molar absorptivity?
Also called the molar extinction coefficient (ε), it is a measure of how strongly a substance absorbs light at a given wavelength. Units: L/(mol·cm).
When does Beer-Lambert law break down?
At high concentrations (above ~0.01 mol/L) due to molecular interactions, or when the light source is not monochromatic.